Effect of StpA concentration on DNase I digestion of supercoiled pBRsk1

Copyright information:Taken from "StpA protein from condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI"Nucleic Acids Research 2005;33(20):6540-6546.Published online 18 Nov 2005PMCID:PMC1289078.© The Author 2005. Published by Oxford University Press. All rights reserved Supercoiled pBRsk1 (CCC) (50 nM) was digested with 0.1 µg/ml DNase I for 1 h at 25°C in high salt EcoKI buffer, pH 7.9. The gel shows undigested DNA (CCC), with digested DNA being absent from the gel, and DNA protected from digestion present as linear (L) and nicked (OC) species. Lane 1 contains linear DNA markers of sizes 2, 3, 4, 5, 6, 8 and 10 kb from bottom to top.