Schematic representation of the microsphere-based high-throughput gene expression assay

Copyright information:Taken from "Design of a Microsphere-Based High-Throughput Gene Expression Assay to Determine Estrogenic Potential"Environmental Health Perspectives 2005;113(9):1164-1171.Published online 12 May 2005PMCID:PMC1280396.This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI. Fluorescent microspheres with covalently bound oligonucleotides specific to the cRNA to be quantified are incubated with cRNA and biotin (b) labeled. Hybridized cRNA is revealed by SAPE [phycoerythrin (PE)-conjugated streptavidin (SA)]. The signal is amplified by a second stain using the biotinylated anti-streptavidin, followed by a third staining step with SAPE.