Reproducibility of the assay, exemplified with the determination of the expression levels of () (up-regulated gene) and () (down-regulated gene) in five independent samples (biologic replicas), in two independent experiments (Rep

Copyright information:Taken from "Design of a Microsphere-Based High-Throughput Gene Expression Assay to Determine Estrogenic Potential"Environmental Health Perspectives 2005;113(9):1164-1171.Published online 12 May 2005PMCID:PMC1280396.This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI. 1 and Rep. 2) with the complete set of samples. Ten micrograms of biotinylated cRNA from control vehicle-treated (control) or EE-exposed tissues (0.1, 1.0, or 10.0 μg/kg/day; low, medium, and high, respectively) was hybridized to microspheres in a five-plex format (with the specific sets for , , , , and ) under the optimized protocol as indicated in “Materials and Methods.” Duplicate results and the means from five samples for each treatment regimen are shown for and . The transcripts are identified in .