Alignment of 25 mtDNA clones in the 452â 528 bp MC molecule of the wheat mitochondrial genome

Copyright information:Taken from "Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome"Nucleic Acids Research 2005;33(19):6235-6250.Published online 31 Oct 2005PMCID:PMC1275586.© The Author 2005. Published by Oxford University Press. All rights reserved Broad, light-green bar shows the MC molecule cleaved between MC coordinates 452 528 and 1. Numbers on the MC molecule show the MC coordinates of the ends of all the clones, their segments and repeat sequences. Rectangle with projection in the broad bar: R1–R9 repeat pairs involved in recombinant clone formation. DRs are dark green, IRs dark brown. The projection shows the direction of each repeat copy. Slender bar: individual clones; light blue, yellow and orange represent intact, single recombinant and double recombinant clones, respectively. L, C or R affixed to clone numbers: Left, central and right segments of a recombinant clone. Note that L and R segments of a single-recombinant clone have the same repeat copy at the end connecting two segments; head-to-tail for DRs, and head-to-head or tail-to-tail for IRs. The double-recombinant clone has a copy of one repeat pair at one end each of its L and C segments and a copy of another repeat pair at the other end of C and at one end of R that, respectively, connect the L and C segments and the C and R segments by recombination.