RecA-catalyzed strand transfer

Copyright information:Taken from "MutS inhibits RecA-mediated strand transfer with methylated DNA substrates"Nucleic Acids Research 2005;33(11):3591-3597.Published online 22 Jun 2005PMCID:PMC1157099.© The Author 2005. Published by Oxford University Press. All rights reserved The top of the figure shows a schematic of the reaction. Single-strand (SS) circular DNA reacts with linear duplex (DS) to form intermediate (I) structures, which are converted to nicked-circle (NC) products. The fluorograph shows the results from the RecA strand transfer assays with unmodified SS DNA and methylated DS DNA (600 μM MNNG treatment) without MutS (left four lanes), with MutS (middle four lanes) and with MutS and MutL (right four lanes). The numbers above the lanes indicate the time of the reaction.