Sequences with a predicted signal peptide from the proteome of , were screened using the algorithm described

Copyright information:Taken from "TMB-Hunt: An amino acid composition based method to screen proteomes for beta-barrel transmembrane proteins"BMC Bioinformatics 2005;6():56-56.Published online 15 Mar 2005PMCID:PMC1274253.Copyright © 2005 Garrow et al; licensee BioMed Central Ltd. Sequences were then sorted by their E-values and plotted graphically. The graph demonstrates that in proteome screening with this tool there a number of sequences will be identified with positive bb scores, but E-values >1. Sequences with these scores are described as being in the twilight zone.