Extended deletions of Oct-4RR N-terminal show no cell specificity for transcriptional activity

Copyright information:Taken from "Use of altered-specificity binding Oct-4 suggests an absence of pluripotent cell-specific cofactor usage"Nucleic Acids Research 2005;33(18):6011-6023.Published online 20 Oct 2005PMCID:PMC1266064.© The Author 2005. Published by Oxford University Press. All rights reserved () Undifferentiated P19 cells or P19 cells induced to differentiate with 0.5 µM RA for 3 days were cotransfected with 250 ng of specified Oct-4RR plasmids and pVκGG11CAT reporter plasmid (reporter plasmid was transfected at 120 ng for P19U and 60 ng for dP19 throughout). p6WCAT reporter plasmid was also used in control transfection experiments were specified. Cells were harvested after 48 h post transfection and whole cell extracts made for CAT assays. CAT assays were subsequently performed on sample extract normalized for β-gal activity within an experiment for each cell type. Figure is representative of data repeated at least three times with averages and standard errors shown. ( and ) In parallel transfection experiments using 1 µg of specified expression vectors, nuclear protein extracts were made and western blots were performed against the translated myc epitope. Blots are representative of duplicated data.