Cloning method used to construct the supervectors by combining the light chain and heavy chain expression vectors

Copyright information:Taken from "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity"Arthritis Research & Therapy 2005;7(5):R971-R983.Published online 10 Jun 2005PMCID:PMC1257422.Copyright © 2005 Mason et al.; licensee BioMed Central Ltd. RI restriction sites in recombinant light chain expression vector, pLN10, containing Vλ cloned DNA sequences. RI-digested light chain cassette containing human cytomegalovirus (HCMV) promoter, immunoglobulin leader sequence, light chain variable-region DNA sequence, and constant-region DNA sequence. Ligation of light chain cassette into RI-linearised B3V/pG1D1 heavy chain vector to produce the final supervector, containing all components required to produce whole IgG1. The four supervectors were constructed in the same way, using the appropriate RI-digested light chain fragments leading to slight variations in the overall plasmid size. SV40, simian virus 40; V:C, variable : constant.