() Depiction of the element InsTet2 and the InsTet1 derived regions for expression integrated into

Copyright information:Taken from "Integrative elements for yielding tetracycline-dependent growth phenotypes"Nucleic Acids Research 2005;33(18):e153-e153.Published online 12 Oct 2005PMCID:PMC1253839.© The Author 2005. Published by Oxford University Press. All rights reserved The promoter P for expression (in WH555) is derived from pWH353, the Pt17 designated promoter (in WH557) is a synthetic construct. () Regulatory capacities of InsTet2 as transcriptional fusion within the loci of WH555 or WH557, yielding WH556 or WH558, respectively. β-Gal measurements are depicted under different growth conditions: open bars indicate no atc, whereas closed bars represent 0.4 µM atc. Values obtained with WH556/pHT304 represent the maximal expression exerted by InsTet2, resulting in Miller units of ∼1200 to ∼1700. Lower panel shows corresponding TetR-directed western blots obtained with the respective soluble proteins. Purified TetR is shown as a control on the left.