DNase I footprinting experiments showing the interaction of TFO-1 and TFO-2 with the intended target site

Copyright information:Taken from "Four base recognition by triplex-forming oligonucleotides at physiological pH"Nucleic Acids Research 2005;33(9):3025-3032.Published online 23 May 2005PMCID:PMC1137030.© The Author 2005. Published by Oxford University Press. All rights reserved TFO-1 generated BAU.AT, MeP.GC, S.TA, PP.CG and T.AT triplets, whereas TFO-2 contains only natural bases and is designed to generate T.AT, C.GC. G.TA and T.CG triplet. The experiments were performed at pH 5.0, 6.0 and 7.0 in appropriate buffers containing 50 mM NaCl. The oligonucleotide concentration (μM) is shown at the top of each gel lane. Tracks labelled ‘GA’ are Maxam–Gilbert markers specific for purines, while ‘con’ indicates DNase I cleavage in the absence of added oligonucleotide. The filled boxes show the position of the triplex target site.