Electrochemical detection of thrombin-dependent proximity-extension-mediated RCA

Copyright information:Taken from "Proximity extension of circular DNA aptamers with real-time protein detection"Nucleic Acids Research 2005;33(6):e64-e64.Published online 7 Apr 2005PMCID:PMC1074748.© The Author 2005. Published by Oxford University Press. All rights reserved () Electroactive nucleoside triphosphate vinyl-Fc-dUTP used in proximity extension assays. () OSWV of DNA SAMs following capture of electroactive proximity extension RCA products at a range of thrombin concentrations. The baseline is a scan of an SAM constructed with a capture oligonucleotide not complementary to RCA products generated at 4 nM thrombin.