Characteristics of the thrombin-dependent proximity extension-mediated RCA reaction

Characteristics of the thrombin-dependent proximity extension-mediated RCA reaction

Copyright information:Taken from "Proximity extension of circular DNA aptamers with real-time protein detection"Nucleic Acids Research 2005;33(6):e64-e64.Published online 7 Apr 2005PMCID:PMC1074748.© The Author 2005. Published by Oxford University Press. All rights reserved () Real-time fl...

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Hauptverfasser: Giusto, Daniel A. Di, Wjatschesslaw A. Wlassoff, J. Justin Gooding, Messerle, Barbara A., King, Garry C.
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Sprache:eng
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Zusammenfassung:Copyright information:Taken from "Proximity extension of circular DNA aptamers with real-time protein detection"Nucleic Acids Research 2005;33(6):e64-e64.Published online 7 Apr 2005PMCID:PMC1074748.© The Author 2005. Published by Oxford University Press. All rights reserved () Real-time fluorescence traces of proximity extension-mediated RCA over a range of thrombin concentrations. () Dependence of maximal RCA rate upon thrombin concentration. Uncorrected rates for 16 pM − 8 nM thrombin (black squares) and background-corrected rates (open circles). The background 0 pM thrombin rate is 1.04 ± 2.3 × 10 arbitrary fluorescence units per second.
DOI:10.6084/m9.figshare.35230