N-terminal SeqA mutant proteins are defective in binding to a pair of hemi-sites, but interact cooperatively

Copyright information:Taken from "Dimeric configuration of SeqA protein bound to a pair of hemi-methylated GATC sequences"Nucleic Acids Research 2005;33(5):1524-1531.Published online 14 Mar 2005PMCID:PMC1065253.© The Author 2005. Published by Oxford University Press. All rights reserved Fractions containing mutant SeqA proteins were incubated with DNA containing a pair of hemi-sites separated by 21 bp (), with DNA containing four hemi-sites with a 16 bp spacing between the pairs of hemi-sites () and DNA containing four hemi-sites with 32 bp spacing (). In each experiment, 33 and 100 ng of wild-type SeqA (designated as WT) and SeqA(E5K), (D7K), (E8K), (E9K) and (E5,9K) partially purified fractions were added to the reaction. The amount of SeqA protein in each case was similar in western blot (data not shown).