The Thr-18 of SeqA contributes to cooperative interaction between SeqA molecules

Copyright information:Taken from "Dimeric configuration of SeqA protein bound to a pair of hemi-methylated GATC sequences"Nucleic Acids Research 2005;33(5):1524-1531.Published online 14 Mar 2005PMCID:PMC1065253.© The Author 2005. Published by Oxford University Press. All rights reserved The binding behavior of purified wild-type SeqA and SeqA(T18G) was analyzed by gel shift experiments: 10, 20 and 40 ng of wild-type SeqA (designated as WT) and SeqA(T18G) were incubated with P-labeled DNA containing a pair of hemi-sites, which were separated by 16 bp (), and two pairs of hemi-sites in which the pairs were separated by 16 bp (), 19 bp () and 41 bp (). Complexes of SeqA and DNA were separated on 5% polyacrylamide gels, dried and visualized by autoradiography.