Optimal conditions for producing adenovirus vector by Cre-mediated RMCE

Copyright information:Taken from "Production of viral vectors using recombinase-mediated cassette exchange"Nucleic Acids Research 2005;33(8):e76-e76.Published online 5 May 2005PMCID:PMC1090444.© The Author 2005. Published by Oxford University Press. All rights reserved The virus stock was prepared on day 5 and then used to infect CV1 cells for a Southern blot analysis. Note that the Ψ-GFP probe was used to detect the specific bands. The results were reproduced in three experiments, and a representative result is shown. () Optimal amount of the donor plasmid DNA. 293FNCre cells were infected with the -expressing recipient virus at an MOI of 2 or 1 and cultured in a 6-well plate, followed by transfection with the indicated amounts of donor plasmid (μg/well). () Optimal MOI of the recipient virus. 293FNCre cells were infected with the -recipient virus at the indicated MOIs, followed by transfection with the donor plasmid at 3.0 μg/well.