A consensus oligonucleotide sequence for binding to hnRNP A2

Copyright information:Taken from "hnRNP A2, a potential ssDNA/RNA molecular adapter at the telomere"Nucleic Acids Research 2005;33(2):486-496.Published online 19 Jan 2005PMCID:PMC548348.© 2005, the authors © () Point mutations were introduced into the dA2RE11 sequence at each position by replacement of the native nucleotide with the other three, separately generating all four oligodeoxyribonucleotides for each position. The ability of the modified oligonucleotides to compete with the unmutated dA2RE11 was assessed by using competition UV-cross-linking EMSAs with recombinant hnRNP A2 in the absence of heparin. Representative results are shown. Controls are shown on the left and the results for the mutations at the indicated positions of the A2RE11 are shown on the right. Competing oligodeoxynucleotides have the point mutations indicated above the autoradiographs. () The wild-type dA2RE11 sequence and single-nucleotide changes that still allow specific binding are shown along with the deduced consensus sequence. In deriving the consensus sequence it has been assumed that substitution at each position is without direct effect on the binding of the non-mutated nucleotides.