ChIP analysis of proteins associated with the gene in nonheat shocked cells

Copyright information:Taken from "Molecular characterization of NELF"Nucleic Acids Research 2005;33(4):1269-1279.Published online 1 Mar 2005PMCID:PMC552961.© The Author 2005. Published by Oxford University Press. All rights reserved Cross-linked chromatin from nonheat shocked cells was subjected to immunoprecipitation with antisera against NELF-B, NELF-E, DSIF or GAGA factor and with corresponding preimmune sera. () Schematic of gene showing regions that were amplified by PCR (not drawn to scale). () Representative results obtained from a ChIP analysis. () Quantification of results obtained from three independent ChIP analyses.