Schematic representation of generating conditional targeting vectors using TnCR7 and TnCR8

Copyright information:Taken from "Transposon-mediated generation of targeting vectors for the production of gene knockouts"Nucleic Acids Research 2005;33(3):e24-e24.Published online 7 Feb 2005PMCID:PMC549422.© The Author 2005. Published by Oxford University Press. All rights reserved TnCR7 is inserted into the target gene by transposition. The gene is deleted using either Cre or a restriction endonuclease, leaving a single copy of LoxP. TnCR8 is then inserted at the other side of the region to be deleted. The construct is now a conditional targeting vector.