Western Blot of HUVEC after treatment with FGF2, infected with the GFP, ca ERK or ca AKT adenoviral constructs, or treated with FGF2 and infected with adenoviral constructs

Copyright information:Taken from "Signalling crosstalk in FGF2-mediated protection of endothelial cells from HIV-gp120"BMC Neuroscience 2005;6():8-8.Published online 2 Feb 2005PMCID:PMC549045.Copyright © 2005 Langford et al; licensee BioMed Central Ltd. The same Western blot was used for all antibodies after stripping and rehybridizing. A) Reacted with anti-phospho ERK antibody reacted with anti-total ERK antibody. Reacted with anti-phospho AKT antibody. Reacted with anti-total AKT antibody. Immuno-complex assay showing changes in ERK activity with or without FGF2 treatment in HUVEC with caERK, caAKT or GFP. Quantification of ERK activity levels in HUVEC +/- FGF2 treatment with caERK, caAKT or GFP with the PhosphorImager as described in the Materials and Methods. * indicates a significant difference from control. * = P < 0.05 by One-Way ANOVA with post-hoc Dunnett's when compared to control.