ASO and siRNA differently influence target mRNA stability and translation

Copyright information:Taken from "Translation of stable hepadnaviral mRNA cleavage fragments induced by the action of phosphorothioate-modified antisense oligodeoxynucleotides"Nucleic Acids Research 2005;33(1):114-125.Published online 07 Jan 2005PMCID:PMC546143.© 2005, the authors © () Schematic diagram of DHBV sgRNA. The initiation codons, hybridization sites for antisense oligos and labeled probes are depicted. The drawing is not to scale. () Northern-blot analysis with probes discriminating the putative mRNA cleavage fragments upon cotransfection of hepatoma cells with a construct encoding sgRNA and different antisense compounds. Equal amounts of RNA were loaded. () Immunoblotting of the concomitantly expressed preS/S proteins, different isoforms are indicated. Equal amounts of protein were loaded.