RNase H activity is not observed in the cytoplasm

Copyright information:Taken from "Translation of stable hepadnaviral mRNA cleavage fragments induced by the action of phosphorothioate-modified antisense oligodeoxynucleotides"Nucleic Acids Research 2005;33(1):114-125.Published online 07 Jan 2005PMCID:PMC546143.© 2005, the authors © () Polyadenylated preS-Luc mRNA was transcribed and capped. The hybridization sites for ASO DHBV795 and labeled probes are depicted. The drawing is not to scale (top). Northern-blot analysis upon cotransfection with ASO DHBV 795 is depicted (bottom). Equal amounts of RNA were loaded. Cotransfected renilla mRNA is shown as control. () Relative luciferase activity obtained after cotransfection of mRNA and ASO DHBV795. Values are given in percent compared to luciferase activity obtained after cotransfection of control ASO. The number of observations is given in brackets. The asterisk indicates a statistically significant difference.