Sequence characteristics of cloned ORFs

Copyright information:Taken from "A genome annotation-driven approach to cloning the human ORFeome"Genome Biology 2004;5(10):R84-R84.Published online 30 Sep 2004PMCID:PMC545604.Copyright © 2004 Collins et al.; licensee BioMed Central Ltd. Plot of the distribution of the 398 chromosome 22 ORFs by GC content (%) and length (bases). Closed circles are the 331 ORFs that were isolated as acceptable clones (278) or as clones with the correct ORF but currently with a problem in the sequence (53). Dotted circles are the rest of the ORFs which were not amplifiable or clonable (67). Overlap of chromosome 22 ORF clones isolated here with cDNA collections. Analysis of GC content and length for 398 chromosome 22 ORFs, split according to whether the gene has been isolated only by the strategy described here (SANGER, red circles), only in the cDNA collections (OTHER, green triangles), in both (BOTH, black circles), or not at all (NOT, yellow triangles).