Activation of neutrophils, eosinophils and macrophages during involution

Copyright information:Taken from "Involution of the mouse mammary gland is associated with an immune cascade and an acute-phase response, involving LBP, CD14 and STAT3"Breast Cancer Research 2003;6(2):R75-R91.Published online 18 Dec 2003PMCID:PMC400652.Copyright © 2004 Stein et al., licens...

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Hauptverfasser: Stein, Torsten, Morris, Joanna S, Davies, Claire R, Weber-Hall, Stephen J, Marie-Anne Duffy, Heath, Victoria J, Bell, Alexandra K, Ferrier, Roderick K, Sandilands, Gavin P, Gusterson, Barry A
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Zusammenfassung:Copyright information:Taken from "Involution of the mouse mammary gland is associated with an immune cascade and an acute-phase response, involving LBP, CD14 and STAT3"Breast Cancer Research 2003;6(2):R75-R91.Published online 18 Dec 2003PMCID:PMC400652.Copyright © 2004 Stein et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. RNA expression for and derived from microarray analysis. RNA expression for , , , , and derived from microarray analysis. The -axes of both graphs describe the different developmental stages: virgin (V10, V12), pregnancy (P1, P2, P3, P8.5, P12.5, P14.5, P17.5), lactation (Lac1, Lac3, Lac7) and involution (Inv1, Inv2, Inv3, Inv4, Inv20). The -axes describe the averaged scaled signal of the triplicate samples for each time point. The intensity data for have been scaled down 10-fold for display. Semi-quantitative RT–PCR for , , and from a fourth independently collected RNA sample. Identification of macrophages, neutrophils and eosinophils. The figure shows the immunohistochemical staining of a paraffin-embedded tissue section from a mouse mammary gland after 4 days of involution. Macrophages were identified by staining with F4/80 antibody and by their nuclear and cellular shapes. Eosinophils were identified by cross-reaction with the F4/80 antibody, polymorphonuclear structure and eosin staining. Neutrophils were identified through their polymorphonuclear structure, clear cytoplasm, slight eosin staining and non-staining with the F4/80 antibody. Cell counts of neutrophils, eosinophils and macrophages present in the mammary tissue during involution. Tissue sections were stained with an antibody against F4/80 and counterstained with H&E; neutrophils and macrophages were counted. Cell counts were taken from 20 (neutrophils and eosinophils) and 50 (macrophages) high-power fields, respectively, from each section in triplicate from three individual mice.
DOI:10.6084/m9.figshare.31288