Effects on bovine collagen type II (BCII)-restimulated spleen-cell cultures from mice fed ovalbumin (OVA)

Copyright information:Taken from "Bystander suppression of collagen-induced arthritis in mice fed ovalbumin"Arthritis Research & Therapy 2004;6(2):R151-R160.Published online 5 Feb 2004PMCID:PMC400434.Copyright © 2004 Bäckström et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. Mice were fed OVA or a standard diet for 7 days. One week after the last day on the OVA diet, the mice were immunized with BCII or BCII mixed with OVA, emulsified in Freund's complete adjuvant. Three weeks later the mice were immunized again, with the same antigen(s) emulsified in Freund's incomplete adjuvant. proliferation , secretion of IFN-γ , IL-4 , and IL-10 by splenocytes after BCII restimulation 1 week after the booster immunization. The proliferation results are presented as proliferation indexes (mean counts per minute [cpm] in triplicate cultures stimulated with OVA/mean cpm in triplicate control cultures). Bars represent mean ± standard error of the mean (8–9). *< 0.05, **< 0.01, ***< 0.001. Ag(s), antigen(s); C, bovine collagen type II; O, ovalbumin; Od, ovalbumin-containing diet; Sd, standard diet.