Similar gene expression patterns of OCI-Ly3 cells treated with flavopiridol, actinomycin D and DRB

Copyright information:Taken from "Genomic-scale measurement of mRNA turnover and the mechanisms of action of the anti-cancer drug flavopiridol"Genome Biology 2001;2(10):research0041.1-research0041.11.Published online 13 Sep 2001PMCID:PMC57796.Copyright © 2001 Lam et al., licensee BioMed Central Ltd OCI-Ly3 cells were treated with flavopiridol (1 μM), actinomycin D (10 μg/ml), or DRB (100 μM) for 0.5, 1, 2, 4 and 8 h. These concentrations were chosen to give roughly equivalent (approximately 50%) cytotoxicity at 24 h without significant loss of cell viability at 8 h. The treated samples were labeled red (Cy5) and untreated samples were labeled green (Cy3). Genes were categorized according to their half-lives by the scheme as described in Materials and methods. Northern blot analysis of selected genes. Comparison of turnover rate of selected genes from microarray data and Northern blot analysis (percentage of control versus time).