Linear amplification of as a large inverted duplication

Genomic organization of the locus in and relative gene expression data as determined by DNA microarrays in MTX20.5. Note that all genes from the telomere up to showed increased levels of expression in the MTX20.5 mutant compared to wild-type cells. Chromosome size PFGE of cells. Ethidium bromide (Et-Br) stained gel, or blotted gels hybridized to a probe or to a probe containing the telomeric repeats are shown. Sizes were determined using a yeast molecular weight marker (Biorad). Model for the formation of the extrachromosomal linear amplicon generated through annealing of homologous inverted repeats (Figure S2 in Additional data file 2). This annealing could be facilitated by a block in replication. PCR with primer pairs 23a and 23b or 23c and 23d to support the model shown in (c). Lane 1, wild-type cells; lane 2, MTX20.5.Copyright information:Taken from "Modulation of gene expression in drug resistant is associated with gene amplification, gene deletion and chromosome aneuploidy"http://genomebiology.com/content/9/7/R115Genome Biology 2008;9(7):R115-R115.Published online 18 Jul 2008PMCID:PMC2530873.