B, C, and E are collapsed images of the z stacks

(A and A′) Cell nuclei (stained with DAPI) in Ttk69-overexpressing follicle cells (A, outlined and green) were much smaller than those in the wild-type cells in a stage 10B egg chamber. (B–B″) A stage 9 egg chamber with Ttk69-overexpressing follicle cells marked by the presence of GFP (B, blue) and high levels of Ttk69 (B, green; B′, white). No or very little BrdU incorporation (B, red; B″, white) was found in these follicle cells (outlined). (C–C″) A control egg chamber with misexpression of GFP (C, blue) but not Ttk69 (C, green; C′, white). The GFP-positive cells (outlined) had normal BrdU incorporation (C, red; C″, white) similar to that of the GFP-negative cells. (D) Quantitative analysis of the BrdU incorporation ratio in the clone area (blue) and outside of the clone (orange) in and egg chambers. In total, 235 Tt69-overexpressing and 703 wild-type follicle cells from three egg chambers were counted. 113 GFP-positive and 236 GFP-negative follicle cells were counted from one egg chamber (C). (E and E′) dMyc (E, red; E′, white) was down-regulated in Ttk69-misexpressing follicle cells in a stage 8 egg chamber. Bars, 10 μm.Copyright information:Taken from "Regulation of the endocycle/gene amplification switch by Notch and ecdysone signaling"The Journal of Cell Biology 2008;182(5):885-896.Published online 8 Sep 2008PMCID:PMC2528591.