Additional file 5 of The Calicophoron daubneyi genome provides new insight into mechanisms of feeding, eggshell synthesis and parasite-microbe interactions

Additional file 5. Comparative histochemical analysis of C. daubneyi and F. hepatica. (A) Tissue sections showing differential staining of adult F. hepatica (Fh) and C. daubneyi (Cd) with Mallory’s Trichrome. In F. hepatica the eggshell protein globule clusters within the vitelline follicles, the eggshell of fully formed eggs within the uterus, and those shed by the flukes in vitro, appeared golden brown whilst the equivalent structures in C. daubneyi stained a deep red colour. Whole mount preparation of adult F. hepatica (B) stained with catechol. The vitelline follicles (arrowed) extend throughout the lateral margins of the fluke and stain golden brown, indicative of tyrosinase. In contrast, in catechol-stained adult C. daubneyi (C) no staining is evident.