Additional file 3 of Dexmedetomidine alleviates intestinal ischemia/reperfusion injury by modulating intestinal neuron autophagy and mitochondrial homeostasis via Nupr1 regulation

Supplementary Material 3: Fig. S3. The Role of Nupr1 Silencing in the Rat Model of I/R. Note: (A) H&E staining for assessment of intestinal tissue damage in rat groups; (B) Utilization of tissue pathology damage score (chiu’s score) for evaluation of severity of intestinal tissue damage in rats; (C-D) Oxidative stress levels in intestinal tissues of various rat groups; (E) Elisa experiment for detection of discrepancies in tnf-α and il-6 levels in rat serum; (F) TUNEL staining for evaluation of apoptotic cell numbers in intestinal tissues of respective rat groups; (G) Immunohistochemistry for determination of proportion of positive cells for lc3b and p62 in intestinal tissues of each rat group; (H) Western blot analysis for quantification of expression levels of Nupr1, LC3, and p62 proteins in intestinal tissues of each rat group; (I) Transmission Electron Microscopy for observation of changes in ultrastructure in intestinal tissues of all groups (Yellow arrows indicate mitochondria, red arrows indicate autophagosomes); (J) DHE probe staining for measurement of ROS Levels. *P