Flow cytometry analysis of hematopoiesis reconstitution

Animal transplanted with autologous CD34bone marrow cells transduced with an SIV-based vector. eGFP-positive cells present in P1 and P2 were analyzed by immuno-staining to identify the subpopulations of eGFP-positive cells in peripheral blood. CD20-PerCP-Cy5, CD14-PE, CD11b-APC and CD3-APC staining were used to identify the B-lymphocyte, monocyte, granulocyte and T-lymphocyte subpopulations.Copyright information:Taken from "Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34bone marrow cells, following gamma irradiation in cynomolgus macaques"http://www.retrovirology.com/content/5/1/50Retrovirology 2008;5():50-50.Published online 19 Jun 2008PMCID:PMC2464606.