Fluorescence microscopy after myeloid differentiation of CFC (×100)

Freshly isolated CD34cells were transduced or not with the lentiviral vector (24 hours of culture with lentiviral vector at MOI = 100). Cells were then cultured for 14 days in the presence of cytokines, to allow myeloid differentiation of transduced (A) and not transduced (B) CD34+ cells. Abbreviati...

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Hauptverfasser: Derdouch, Sonia, Gay, Wilfried, Nègre, Didier, Prost, Stéphane, Dantec, Mikael Le, Delache, Benoît, Auregan, Gwenaelle, Andrieu, Thibault, Jean-Jacques Leplat, François-Loïc Cosset, Grand, Roger Le
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Sprache:eng
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Zusammenfassung:Freshly isolated CD34cells were transduced or not with the lentiviral vector (24 hours of culture with lentiviral vector at MOI = 100). Cells were then cultured for 14 days in the presence of cytokines, to allow myeloid differentiation of transduced (A) and not transduced (B) CD34+ cells. Abbreviations: CFU-GEMM, Colony-Forming Unit-Granulocytes, Erythroid, Macrophage, Megakaryocyte; BFU-E, Burst-Forming Unit-Erythroid; CFU-GM, Colony-Forming Unit-Granulocytes, Macrophage; CFU-G, Colony-Forming Unit-Granulocytes; CFU-M, Colony-Forming Unit-Macrophage.Copyright information:Taken from "Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34bone marrow cells, following gamma irradiation in cynomolgus macaques"http://www.retrovirology.com/content/5/1/50Retrovirology 2008;5():50-50.Published online 19 Jun 2008PMCID:PMC2464606.
DOI:10.6084/m9.figshare.27508