Additional file 3 of IL-6 deficiency accelerates cerebral cryptococcosis and alters glial cell responses

Additional file 3 of IL-6 deficiency accelerates cerebral cryptococcosis and alters glial cell responses

Supplementary Material 3: siRNA treatment does not affect NR-9460 microglia-like cell viability. (A) Representative flow cytometry dot plots for untreated and siRNA IL-6- or siRNA negative control-treated NR-9460 cells are shown. Cells were stained with 7-AAD for viability after a 24 h siRNA treatme...

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Bibliographische Detailangaben
Hauptverfasser: Reguera-Gomez, Marta, Munzen, Melissa E., Hamed, Mohamed F., Charles-Niño, Claudia L., Martinez, Luis R.
Format: Bild
Sprache:eng
Schlagworte:
Cancer
Ecology
FOS: Biological sciences
FOS: Clinical medicine
FOS: Health sciences
Immunology
Infectious Diseases
Medicine
Mental Health
Microbiology
Neuroscience
Physiology
Plant Biology
Virology
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Zusammenfassung:Supplementary Material 3: siRNA treatment does not affect NR-9460 microglia-like cell viability. (A) Representative flow cytometry dot plots for untreated and siRNA IL-6- or siRNA negative control-treated NR-9460 cells are shown. Cells were stained with 7-AAD for viability after a 24 h siRNA treatment at 37°C and 5% CO2. Each plot was generated after ≥ 10,000 events were analyzed. (B) The percentage of microglia-like cell viability was determined. Each symbol represents an independent replicate (n = 4). Bars and error bars denote means and SDs, respectively. Significance (*, P 
DOI:10.6084/m9.figshare.27123875