Additional file 11 of Repeat length of C9orf72-associated glycine–alanine polypeptides affects their toxicity

Additional file 11. Figure S11: Genetic interventions that target autophagy, the proteasome, the unfolded protein response and chaperones partially rescue GA400-induced toxicity during development. A Representative eye images of female flies co-expressing GA400 and the indicated constructs using GMR-Gal4. B Eye size of flies normalized to the mean of the eye size of GMR-Gal4/+ control flies. Co-expression of ATG1and p62 partially rescued the reduced eye size of GA400 expressing flies. Knock down of Hsp22, Hsp83 and p62 via RNAi and expression of hUNC119 reduced the eye size of GA400 flies (One-Way ANOVA + Dunnett’s multiple corrections test; n = 3-10 fly eyes; ***P < 0.001, ***P < 0.001, ***P < 0.0001). C-D Protein levels of C Hsp22 and D Hsp83 measured by mass spectrometry-based proteomics in female flies that expressed GA100, GA200 or GA400. Data are shown after z-score normalization. Hsp22 and Hsp83 were up-regulated by short-term expression of GA400 and after prolonged expression of GA100 and GA200 (One-Way ANOVA + Tukey’s multiple corrections test; n = 3-4 replicates of 25 fly brains; **P < 0.01, ***P < 0.001, ***P < 0.0001).