Additional file 7 of Repeat length of C9orf72-associated glycine–alanine polypeptides affects their toxicity

Additional file 7. Figure S7: Validation of fly lines carrying GA400-GFP and GA400-mCherry constructs. A Schematic overview of UAS-GA400-GFP and UAS-GA400-mCherry constructs. Brown arrows indicate the location of primers used for the PCR-based genotyping in B. U: indicates the size of the uncut DNA amplicon and D: indicates the size of the DNA amplicon after XbaI/NotI restriction. B PCR-based genotyping of three independently generated transgenic fly lines (#1-3) carrying an insertion of UAS-GA400-GFP and UAS-GA400-mCherry in the attP2 landing site confirmed full-length integration of the tagged polyGA transgenes. Flies only carrying the elav-GS driver construct were used as a negative control. Unless indicated otherwise, transgenic fly line #1 was used for all experiments.