Supplementary Fig. 1. Flow cytometry gating strategy for dot plots of representative samples shown in Figure 1D

Flow cytometry gating strategy for dot plots of representative samples shown in Figure 1D. The upper plots (1st row) display the annexin V-FITC and PI fluorescence for the entire dataset with the double-negative (DN) region indicated. The lower plots (2nd row) show FSC vs. SSC plots gated on the DN cells, highlighting the larger population of true live cells (annexin V-FITC negative, PI negative, and large) and the other population identified as debris. This debris gate was then inverted (i.e., excluding debris) and applied to the annexin V-FITC vs. PI plot.