Projections of MO pyrCWNPs-S3

Video S3. (Microbiology Spectrum Spectrum00645-24). 3D projections of A. infectoria pyrCWNPs, in RAW 264.7 macrophages. Macrophages were exposed during 6 h to CWNPs of A. infectoria grown with 50 µg/mL pyroquilon (pyrCWNPs). The CWNPs were labelled with CFW (Fluorescent Brightener 28, Sigma®), a probe for fungal cell wall chitin, and macrophage was labelled with LysoTracker Red® (Red DND-99, Invitrogen), a fluorescent probe that responds to acidic intracellular compartments. Molecular Probes®). Scale bar indicates 100 pixels. The cell imaging was performed on a Carl Zeiss LSM 710 Confocal Microscope, using a 63x PlanApoChromat (NA 1.4) oil objective and Z-stack experiment with the analysis was achieved using Fiji software, with Plugging Cell Counter and 3D Viewer. 3D projections were obtained from reconstruction of 15 frames.