Engineering bacteria to solve the Burnt Pancake Problem-5

R (blue arrow) and (green rectangle = , purple arrow = ). The promoter is pancake 1 and is pancake 2. sites (yellow rectangles) flank each flippable element. Inversion of is detected by expression of the reverse (rev) upstream -reporter. HinLVA is expressed from a second plasmid (right). AmpR = ampi...

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Hauptverfasser: Karmella A Haynes, Broderick, Marian L, Brown, Adam D, Butner, Trevor L, Dickson, James O, W Lance Harden, Heard, Lane H, Jessen, Eric L, Malloy, Kelly J, Ogden, Brad J, Sabriya Rosemond, Simpson, Samantha, Zwack, Erin, A Malcolm Campbell, Eckdahl, Todd T, Heyer, Laurie J, Poet, Jeffrey L
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Zusammenfassung:R (blue arrow) and (green rectangle = , purple arrow = ). The promoter is pancake 1 and is pancake 2. sites (yellow rectangles) flank each flippable element. Inversion of is detected by expression of the reverse (rev) upstream -reporter. HinLVA is expressed from a second plasmid (right). AmpR = ampicillin resistance marker, ChlrR = chloramphenicol resistance marker, repA pSC101 and ColE1 = origins of replication, RBS = ribosome binding site, TT = double transcription terminator, white boxes = cloning sites.Copyright information:Taken from "Engineering bacteria to solve the Burnt Pancake Problem"http://www.jbioleng.org/content/2/1/8Journal of Biological Engineering 2008;2():8-8.Published online 20 May 2008PMCID:PMC2427008.
DOI:10.6084/m9.figshare.26776