Engineering bacteria to solve the Burnt Pancake Problem-3

Owed by ---is shown at the top. In this arrangement, cannot drive expression. Simultaneous inversion of both segments converts into ; the promoter is directed towards so that expression is turned on. (B, inset C) White light photograph of colonies ~18-hours after cotransformation with BPP plasmid an...

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Hauptverfasser: Karmella A Haynes, Broderick, Marian L, Brown, Adam D, Butner, Trevor L, Dickson, James O, W Lance Harden, Heard, Lane H, Jessen, Eric L, Malloy, Kelly J, Ogden, Brad J, Sabriya Rosemond, Simpson, Samantha, Zwack, Erin, A Malcolm Campbell, Eckdahl, Todd T, Heyer, Laurie J, Poet, Jeffrey L
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creator Karmella A Haynes
Broderick, Marian L
Brown, Adam D
Butner, Trevor L
Dickson, James O
W Lance Harden
Heard, Lane H
Jessen, Eric L
Malloy, Kelly J
Ogden, Brad J
Sabriya Rosemond
Simpson, Samantha
Zwack, Erin
A Malcolm Campbell
Eckdahl, Todd T
Heyer, Laurie J
Poet, Jeffrey L
description Owed by ---is shown at the top. In this arrangement, cannot drive expression. Simultaneous inversion of both segments converts into ; the promoter is directed towards so that expression is turned on. (B, inset C) White light photograph of colonies ~18-hours after cotransformation with BPP plasmid and HinLVA. (D, inset E) mRFP protein production visualized under ultraviolet light (solid white arrow) indicates a simultaneous flip of two adjacent pancakes that placed in the reverse orientation adjacent to -. Some colonies do not glow red (open arrow), indicating a lack of double segment flipping or subsequent conversion into other arrangements that lack expression (, ).Copyright information:Taken from "Engineering bacteria to solve the Burnt Pancake Problem"http://www.jbioleng.org/content/2/1/8Journal of Biological Engineering 2008;2():8-8.Published online 20 May 2008PMCID:PMC2427008.
doi_str_mv 10.6084/m9.figshare.26774
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title Engineering bacteria to solve the Burnt Pancake Problem-3
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