Engineering bacteria to solve the Burnt Pancake Problem-3

Owed by ---is shown at the top. In this arrangement, cannot drive expression. Simultaneous inversion of both segments converts into ; the promoter is directed towards so that expression is turned on. (B, inset C) White light photograph of colonies ~18-hours after cotransformation with BPP plasmid an...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Karmella A Haynes, Broderick, Marian L, Brown, Adam D, Butner, Trevor L, Dickson, James O, W Lance Harden, Heard, Lane H, Jessen, Eric L, Malloy, Kelly J, Ogden, Brad J, Sabriya Rosemond, Simpson, Samantha, Zwack, Erin, A Malcolm Campbell, Eckdahl, Todd T, Heyer, Laurie J, Poet, Jeffrey L
Format: Bild
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Owed by ---is shown at the top. In this arrangement, cannot drive expression. Simultaneous inversion of both segments converts into ; the promoter is directed towards so that expression is turned on. (B, inset C) White light photograph of colonies ~18-hours after cotransformation with BPP plasmid and HinLVA. (D, inset E) mRFP protein production visualized under ultraviolet light (solid white arrow) indicates a simultaneous flip of two adjacent pancakes that placed in the reverse orientation adjacent to -. Some colonies do not glow red (open arrow), indicating a lack of double segment flipping or subsequent conversion into other arrangements that lack expression (, ).Copyright information:Taken from "Engineering bacteria to solve the Burnt Pancake Problem"http://www.jbioleng.org/content/2/1/8Journal of Biological Engineering 2008;2():8-8.Published online 20 May 2008PMCID:PMC2427008.
DOI:10.6084/m9.figshare.26774