Additional file 3 of Endometrial senescence is mediated by interleukin 17 receptor B signaling

Additional file 3: Fig. S3. (A-B) SPiDER-β-Gal flow cytometry of endometrial organoids. Horizontal axis: SPiDER-β-Gal intensity; vertical axis: unit area. Bar graphs show the percentage of the area under each curve that was brighter than in the negative control (yellow area: SA-β-gal-positive cells). (A) EM_3, passage 2. (B) EM_4, passage 1. (C-D) p21 mRNA expression in endometrial organoids cultured in ExM containing IL1β (0, 10, 100 ng/ml). Expression was quantified via RT-qPCR relative to an endogenous control (18S rRNA). Error bars denote standard deviation (n = 3). (C) EM_2, passages 0, 3. (D) EM_5, passages 0, 3. * P < 0.05, ** P < 0.01.