Additional file 1 of G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis

Supplementary Material 1: (A) The viability of in A549 and H1299 cells overexpressing GPER1 and the vector was determined using the CCK8 assay after 48 h of treatment with H2O2. (B) The viability of GPER1 knockdown and negative control A549 and H1299 cells was determined using the CCK8 assay after 48 h of treatment with H2O2. (C) The dose-viability curve revealed that GPER1 reduced sensitivity to the ferroptosis inducers RSL3 and Erastin. (D) GPER1, p-PI3K, p-AKT and p-mTOR expression in subcutaneous tumors in the GPER1-knockdown and control groups were determined using immunohistochemistry. The results are presented as the mean ± SD. n = 3; *P