Additional file 2 of The microRNA-211-5p/P2RX7/ERK/GPX4 axis regulates epilepsy-associated neuronal ferroptosis and oxidative stress

Additional file 2: Figure S1. A The transcriptome data of GSE99455 abstracted from hippocampal miRNA profiling of intractable epilepsy and healthy controls. MiR-211-5p was downregulated in adult-onset epilepsy patients vs. controls. B MiR-211-5p was downregulated in adolescence-onset epilepsy patients vs. controls. C the RNA sequence alignment showed that the 3′-UTR of P2RX7 mRNA contained a complementary site for the seed region of miRNA-211-5p. D The relative mRNA levels of miRNA-211-5p in mimic and mimic nc groups for 50 nm/24 h, 100 nm/24 h, 50 nm/48 h, 100 nm/48 h were measured by qRT-PCR. E The relative mRNA levels of miRNA-211-5p in inhibitor and inhibitor nc groups for 50 nm/24 h, 100 nm/24 h, 50 nm/48 h, 100 nm/48 h were measured by qRT-PCR. F, G Western blots and quantification of the protein levels of P2RX7 in the HT22 and SH-SY5Y cells. H The relative mRNA levels of P2RX7 in siRNA-P2RX7 and siRNA-NC groups for 50 nm/24 h, 100 nm/24 h, 50 nm/48 h, 100 nm/48 h were measured by qRT-PCR. I Enrichment analysis was performed utilizing the DEGs extracted from seven distinct cellular subgroups (GO terms). All data are expressed as the mean ± SD. *p