Additional file 5 of CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p/CYP1B1 axis

Additional file 5: Figure S5. CircPPAP2B regulates HNRNPC nondegradable ubiquitination via TRIM25 and USP10. A) Protein half-life assays were performed to explore the role of circPPAP2B on HNRNPC expression. B) Western blotting assays were performed to explore the role of circPPAP2B knockdown or overexpression on HNRNPC expression. C) CoIP assay was performed to explore whether circPPAP2B regulates the ubiquitination of HNRNPC in 293T cells. D) CoIP assay was performed to determine the role of circPPAP2B on the interaction between HNRNPC and USP10. E) The predicted 3D model of HNRNPC/TRIM25/USP10 by HDOCK software. F) CoIP assay was performed to explore whether circPPAP2B regulates the interaction between HNRNPC and TRIM25 or USP10. G) CoIP assay was performed to determine the role of TRIM25 on ubiquitination levels of HNRNPC. H) CoIP assay was performed to determine the role of TRIM25 on the interaction of HNRNPC with Importin α7.