Additional file 6 of GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway

Additional file 6: Figure. S6. GABA promotes the proliferation and migration of CRC by inhibiting ubiquitination of cMYC through miR-223-3p. (A) The proliferation of colon cancer cells transfected with miR-mimic or negative control was assessed via CCK8 for 3 days. (B) The proliferation of colon cancer cells transfected with miR-mimic or negative control was assessed via colony formation assay for 10 days. (C) The wound healing assays showed that overexpression of miR-223-3p augmented the migrative ability of colon cancer cells. (D) The transwell assays indicated that overexpression of miR-223-3p augmented the migrative ability of colon cancer cells. (E) qRT-PCR showed that the expression of miR-223-3p was significantly increased when overexpressed cMYC in LoVo cells. (F) qRT-PCR showed that the expression of miR-223-3p was significantly decreased when knocked down cMYC in LoVo cells. (G) The schematic diagram exhibited one predicted binding site between cMYC and the miR-223-3p promoter. (H) Chip assays with cMYC antibody or IgG were performed to verify binding between cMYC and the miR-223-3p promoter in 293T cells. All data were revealed as mean ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P