Additional file 5 of Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
Additional file 5: Figure S5. Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4w...
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| creator | Nguyen, Linda T. Aprico, Andrea Nwoke, Eze Walsh, Alexander D. Blades, Farrah Avneri, Raphael Martin, Elodie Zalc, Bernard Kilpatrick, Trevor J. Binder, Michele D. |
| description | Additional file 5: Figure S5. Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4wk CPZ and 5wk CPZ. After cuprizone-challenge, cohorts of mice were returned to standard chow for tissue collection at remyelination time-points: 4wk CPZ + 2wk rec and 5wk CPZ + 2wk rec. Corpus callosum tissue was dissected and processed for RNA extraction and qPCR. B Dissected corpus callosum tissue from cKO and WT animals displayed no differences in Ifng gene expression gene expression at any time-point (P > 0.05). Relative gene expression presented on log2 scale. n = 3–5 biological replicates. All data represent mean ± SD. Data from 3 to 5 weeks CPZ time-points analysed by unpaired t-tests. Data from 2–3 weeks recovery time-points analysed by Welch’s t test. |
| doi_str_mv | 10.6084/m9.figshare.26636100 |
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Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4wk CPZ and 5wk CPZ. After cuprizone-challenge, cohorts of mice were returned to standard chow for tissue collection at remyelination time-points: 4wk CPZ + 2wk rec and 5wk CPZ + 2wk rec. Corpus callosum tissue was dissected and processed for RNA extraction and qPCR. B Dissected corpus callosum tissue from cKO and WT animals displayed no differences in Ifng gene expression gene expression at any time-point (P > 0.05). Relative gene expression presented on log2 scale. n = 3–5 biological replicates. All data represent mean ± SD. Data from 3 to 5 weeks CPZ time-points analysed by unpaired t-tests. 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Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4wk CPZ and 5wk CPZ. After cuprizone-challenge, cohorts of mice were returned to standard chow for tissue collection at remyelination time-points: 4wk CPZ + 2wk rec and 5wk CPZ + 2wk rec. Corpus callosum tissue was dissected and processed for RNA extraction and qPCR. B Dissected corpus callosum tissue from cKO and WT animals displayed no differences in Ifng gene expression gene expression at any time-point (P > 0.05). Relative gene expression presented on log2 scale. n = 3–5 biological replicates. All data represent mean ± SD. Data from 3 to 5 weeks CPZ time-points analysed by unpaired t-tests. Data from 2–3 weeks recovery time-points analysed by Welch’s t test.</description><subject>Cancer</subject><subject>Cell Biology</subject><subject>Developmental Biology</subject><subject>FOS: Biological sciences</subject><subject>FOS: Clinical medicine</subject><subject>Genetics</subject><subject>Hematology</subject><subject>Immunology</subject><subject>Medicine</subject><subject>Molecular Biology</subject><subject>Neuroscience</subject><subject>Pharmacology</subject><subject>Virology</subject><fulltext>true</fulltext><rsrctype>image</rsrctype><creationdate>2024</creationdate><recordtype>image</recordtype><sourceid>PQ8</sourceid><recordid>eNqdjj0KAjEQRtNYiHoDi7mAa9afRUtZFBtttA9hM1kHJ8mSRNDbu4JewOrj4_HgCTEtZVHJzWrutoWlNt10xGJRVcuqlHIoeGcMZQpeM1hihDUECyeM-T7DZxcxJfItOGpiaJk0kLf8QN8gBKY2GPSmJ-gxUQLtDbgXMnlwPWL-uP3JN4T6fBmLgdWccPLdkVgd9tf6ODM664Yyqi6S0_GlSqk-0cpt1S9a_aKXf2pvpctW5Q</recordid><startdate>20240814</startdate><enddate>20240814</enddate><creator>Nguyen, Linda T.</creator><creator>Aprico, Andrea</creator><creator>Nwoke, Eze</creator><creator>Walsh, Alexander D.</creator><creator>Blades, Farrah</creator><creator>Avneri, Raphael</creator><creator>Martin, Elodie</creator><creator>Zalc, Bernard</creator><creator>Kilpatrick, Trevor J.</creator><creator>Binder, Michele D.</creator><general>figshare</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20240814</creationdate><title>Additional file 5 of Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS</title><author>Nguyen, Linda T. ; Aprico, Andrea ; Nwoke, Eze ; Walsh, Alexander D. ; Blades, Farrah ; Avneri, Raphael ; Martin, Elodie ; Zalc, Bernard ; Kilpatrick, Trevor J. ; Binder, Michele D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_6084_m9_figshare_266361003</frbrgroupid><rsrctype>images</rsrctype><prefilter>images</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cancer</topic><topic>Cell Biology</topic><topic>Developmental Biology</topic><topic>FOS: Biological sciences</topic><topic>FOS: Clinical medicine</topic><topic>Genetics</topic><topic>Hematology</topic><topic>Immunology</topic><topic>Medicine</topic><topic>Molecular Biology</topic><topic>Neuroscience</topic><topic>Pharmacology</topic><topic>Virology</topic><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Linda T.</creatorcontrib><creatorcontrib>Aprico, Andrea</creatorcontrib><creatorcontrib>Nwoke, Eze</creatorcontrib><creatorcontrib>Walsh, Alexander D.</creatorcontrib><creatorcontrib>Blades, Farrah</creatorcontrib><creatorcontrib>Avneri, Raphael</creatorcontrib><creatorcontrib>Martin, Elodie</creatorcontrib><creatorcontrib>Zalc, Bernard</creatorcontrib><creatorcontrib>Kilpatrick, Trevor J.</creatorcontrib><creatorcontrib>Binder, Michele D.</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Nguyen, Linda T.</au><au>Aprico, Andrea</au><au>Nwoke, Eze</au><au>Walsh, Alexander D.</au><au>Blades, Farrah</au><au>Avneri, Raphael</au><au>Martin, Elodie</au><au>Zalc, Bernard</au><au>Kilpatrick, Trevor J.</au><au>Binder, Michele D.</au><format>book</format><genre>unknown</genre><ristype>GEN</ristype><title>Additional file 5 of Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS</title><date>2024-08-14</date><risdate>2024</risdate><abstract>Additional file 5: Figure S5. Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4wk CPZ and 5wk CPZ. After cuprizone-challenge, cohorts of mice were returned to standard chow for tissue collection at remyelination time-points: 4wk CPZ + 2wk rec and 5wk CPZ + 2wk rec. Corpus callosum tissue was dissected and processed for RNA extraction and qPCR. B Dissected corpus callosum tissue from cKO and WT animals displayed no differences in Ifng gene expression gene expression at any time-point (P > 0.05). Relative gene expression presented on log2 scale. n = 3–5 biological replicates. All data represent mean ± SD. Data from 3 to 5 weeks CPZ time-points analysed by unpaired t-tests. Data from 2–3 weeks recovery time-points analysed by Welch’s t test.</abstract><pub>figshare</pub><doi>10.6084/m9.figshare.26636100</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Cancer Cell Biology Developmental Biology FOS: Biological sciences FOS: Clinical medicine Genetics Hematology Immunology Medicine Molecular Biology Neuroscience Pharmacology Virology |
| title | Additional file 5 of Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS |
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