Additional file 5 of Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS

Additional file 5: Figure S5. Ifnγ gene expression is not altered by loss of microglial Mertk during cuprizone-mediated demyelination or subsequent remyelination. A Demyelination was induced in Mertk WT and cKO using cuprizone (0.2% w/w). Mice were collected at demyelination time-points: 3wk CPZ, 4wk CPZ and 5wk CPZ. After cuprizone-challenge, cohorts of mice were returned to standard chow for tissue collection at remyelination time-points: 4wk CPZ + 2wk rec and 5wk CPZ + 2wk rec. Corpus callosum tissue was dissected and processed for RNA extraction and qPCR. B Dissected corpus callosum tissue from cKO and WT animals displayed no differences in Ifng gene expression gene expression at any time-point (P > 0.05). Relative gene expression presented on log2 scale. n = 3–5 biological replicates. All data represent mean ± SD. Data from 3 to 5 weeks CPZ time-points analysed by unpaired t-tests. Data from 2–3 weeks recovery time-points analysed by Welch’s t test.