Additional file 2 of The novel HS-mimetic, Tet-29, regulates immune cell trafficking across barriers of the CNS during inflammation

Additional file 2: Figure S2. Gating strategies for flow cytometry. (a) Cells of interest were identified by forward scatter (FSC) and side scatter (SSC) parameters, and CD45 expression. (b) Microglia and leukocytes were differentiated based on their CD45 expression, and there was no significant dif...

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Hauptverfasser: Peck, Tessa, Davis, Connor, Lenihan-Geels, Georgia, Griffiths, Maddie, Spijkers-Shaw, Sam, Zubkova, Olga V., La Flamme, Anne Camille
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Sprache:eng
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Zusammenfassung:Additional file 2: Figure S2. Gating strategies for flow cytometry. (a) Cells of interest were identified by forward scatter (FSC) and side scatter (SSC) parameters, and CD45 expression. (b) Microglia and leukocytes were differentiated based on their CD45 expression, and there was no significant difference in microglia counts in the brain between disease or treatment groups. Data are pooled from 4 independent experiments and displayed as mean ± SEM (n = 12–18 per group). (c) Gating strategy to identify lymphocytes from single-cell, CD45+ populations. (d) Gating strategy to identify myeloid cells from single-cell, CD45+ populations.
DOI:10.6084/m9.figshare.26634591