Additional file 7 of UGDH promotes tumor-initiating cells and a fibroinflammatory tumor microenvironment in ovarian cancer

Additional file 7: Supplementary Figure 2. Effects of UGDH knockdown in OV90 cells, and over-expression in ACI23 cells in vitro. A) Representative brightfield images of adherent cell culture morphology of OV90 control (shneg) and UGDH knockdown (sh459, sh939) cells and B) ACI23 control (VC) and UGDH...

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Hauptverfasser: Harrington, Brittney S., Kamdar, Rahul, Ning, Franklin, Korrapati, Soumya, Caminear, Michael W., Hernandez, Lidia F., Butcher, Donna, Edmondson, Elijah F., Traficante, Nadia, Hendley, Joy, Gough, Madeline, Rogers, Rebecca, Lourie, Rohan, Shetty, Jyoti, Tran, Bao, Elloumi, Fathi, Abdelmaksoud, Abdalla, Nag, Madhu Lal, Mazan-Mamczarz, Krystyna, House, Carrie D., Hooper, John D., Annunziata, Christina M.
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Sprache:eng
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Zusammenfassung:Additional file 7: Supplementary Figure 2. Effects of UGDH knockdown in OV90 cells, and over-expression in ACI23 cells in vitro. A) Representative brightfield images of adherent cell culture morphology of OV90 control (shneg) and UGDH knockdown (sh459, sh939) cells and B) ACI23 control (VC) and UGDH-overexpressing (OverX) cells. Magnifications as indicated; scale bar is 100µm. C) Representative images of OV90 control cells (shneg) and UGDH silenced cells (sh459, 939) with immunofluorescent staining of E-cadherin (green), Vimentin (purple), and merged images with nuclear stain DAPI (blue) at 60x magnification, scale bar is 20µm. D) Ratio of E-cadherin: Vimentin intensity of immunofluorescent images. *p
DOI:10.6084/m9.figshare.26628913