Additional file 3 of Novel methodologies for host-microbe interactions and microbiome-targeted therapeutics in 3D organotypic skin models

Additional file 3: Supplemental Figure S3. Inoculum and stratum corneum thickness do not influence growth of S. aureus ATCC 29213. (A) Colony forming unit (CFU) count of HEEs inoculated with a concentration series (101, 102, 103, 104, 106 and 107 CFU) of S. aureus and harvested after 20, 28 and 44 hours of culture (N=2). (B) Normal epidermal protein expression after S. aureus colonization up to 13 days compared to the control HEE (PBS) shown with the proliferation marker Ki-67 and the differentiation markers filaggrin (FLG) and involucrin (IVL). (C) CFU analysis of S. aureus colonized at day 8 and day 11 (thick layer of stratum corneum) of the air-liquid interface (ALI) for 24 hours (biological N=5, input at day 0). (D) SKALP/elafin protein expression of HEE inoculated with S. aureus at day 11 of the ALI (thick layer of stratum corneum) in comparison with the control HEE (PBS) and cultured for 24 hours. Images represent N=5 biological keratinocyte donors. Scale bar = 100 µm.