Additional file 4 of GMP-grade human neural progenitors delivered subretinally protect vision in rat model of retinal degeneration and survive in minipigs

Additional file 4: Fig S1. hNPC and CNS10-NPC preserve vision in a dose-response fashion. A and B Optokinetic response (OKR) shows that hNPC-treated groups have significantly higher spatial visual acuity in the optimal dose (60 K) compared with other groups at both P60 and P90 timepoints. There is no significant difference between the optimal dose and higher dose groups (n=3 for vehicle treated, n=6 for 6 K dose, n=9 for 20 K-400 K doses, n=45 for untreated eyes). C and D Photopic electroretinography (ERG) shows that the optimal dose offered significantly higher b-wave amplitude compared with low dose and control groups, with no significant difference among the three high doses. E Spectral Domain Optical Coherence Tomography (SD-OCT) was performed at P60 and P90 following cell or vehicle injection at P21-23. At P60, ONL is visible from all the groups, but is clearly thicker in the 60K-400K hNPC groups. Lumps (Arrows showing cells in 200 K and 400 K were detected in retinas with 60 K or higher treatment at P60 associated with thicker ONL, which were largely flatten out at P90). The ONL in lower dose and controls was no longer visible, while in 60 K or higher dose treated groups, ONL was clearly visible at P90. Scale bar = 400 µm. Data are represented as mean ± SEM. One-way ANOVA with Tukey’s test was used for multiple comparisons. *p